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| Covizi,D.Z.; Carvalho,H.F.. |
| The structure of the large proteoglycan present in the bullfrog epiphyseal cartilage was studied by immunochemical and biochemical methods. The isolated monomer showed a polydisperse behavior on Sepharose CL2B, with a peak at Kav = 0.14. Chondroitin sulfate chains were identified by HPLC analysis of the products formed by chondroitinase digestion and mercuric acetate treatment. These chains have approximately 38 disaccharides, a Di45:Di68 ratio of 1.6 and GalNAc4S + GalNAc4,6S are the main non-reducing terminals. Keratan sulfate was identified by the use of two monoclonal antibodies in Western blots after chondroitinase ABC treatment. A keratan sulfate-rich region (~110 kDa) was isolated by sequential treatment with chondroitinase ABC and proteases. We... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aggrecan; Bullfrog; Cartilage; Chondroitin sulfate; Epiphyseal cartilage. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200002 |
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| Berto,A.G.A.; Oba,S.M.; Michelacci,Y.M.; Sampaio,L.O.. |
| In many tumors, the amount of chondroitin sulfate in the extracellular matrix has been shown to be elevated when compared to the corresponding normal tissue. Nevertheless, the degree of chondroitin sulfate increase varies widely. In order to investigate a possible correlation between the amount of chondroitin sulfate and tumor size, several individual specimens of human leiomyoma, a benign uterine tumor, were analyzed. The glycosaminoglycans from eight tumors were extracted and compared with those from the respective adjacent normal myometrium. The main glycosaminoglycan found in normal myometrium was dermatan sulfate, with small amounts of chondroitin sulfate and heparan sulfate. In leiomyoma, both dermatan sulfate and chondroitin sulfate were detected... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Galactosaminoglycan; Glycosaminoglycan; Chondroitin sulfate; Dermatan sulfate; Leiomyoma; Myometrium. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500011 |
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| Moreira,Juliana Junqueira; Coelho,Joyce Martins; Machado,Thais Sodré Lima; Morais,Ana Paula Lopes; Michelacci,Yara Maria; Baccarin,Raquel Yvonne Arantes. |
| ABSTRACT: Several studies, mainly in vitro, have shown that chondroitin sulfate (CS) and glucosamine (GlcN) do have chondro protective and anti-inflammatory actions. The aim of the present study was to investigate whether oral CS/GlcN supplementation has effects on the CS, hyaluronic acid (HA) and prostaglandin E2 (PGE2) concentrations on synovial fluid of equine osteoarthritic joints. Horses with mild osteoarthritis (OA) in tibiotarsal joint received daily PO doses of CS and GlcN (2.8/3.1 g) for 25 days. Synovial fluid (SF) and urine samples were collected before treatment (day 0), and every 7 days, until day 55 (30 days after the end of treatment). Urinary CS increased upon oral treatment, indicating that this compound was systemically distributed.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chondroitin sulfate; Horse; Glucosamine; Osteoarthritis; Synovial fluid. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000900602 |
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| Aguiar,J.A.K.; Michelacci,Y.M.. |
| Flavobacterium heparinum is a soil bacterium that produces several mucopolysaccharidases such as heparinase, heparitinases I and II, and chondroitinases AC, B, C and ABC. The purpose of the present study was to optimize the preparation of F. heparinum chondroitinases, which are very useful tools for the identification and structural characterization of chondroitin and dermatan sulfates. We observed that during the routine procedure for cell disruption (ultrasound, 100 kHz, 5 min) some of the chondroitinase B activity was lost. Using milder conditions (2 min), most of the chondroitinase B and AC protein was solubilized and the enzyme activities were preserved. Tryptic soy broth without glucose was the best culture medium both for bacterial growth and enzyme... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chondroitinases; Mucopolysaccharidases; Dermatan sulfate; Chondroitin sulfate. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500007 |
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| Cavalcante,L.A.; Garcia-Abreu,J.; Mendes,F.A.; Moura Neto,V.; Silva,L.C.F.; Onofre,G.; Weissmüller,G.; Carvalho,S.L.. |
| Proteoglycans are abundant in the developing brain and there is much circumstantial evidence for their roles in directional neuronal movements such as cell body migration and axonal growth. We have developed an in vitro model of astrocyte cultures of the lateral and medial sectors of the embryonic mouse midbrain, that differ in their ability to support neuritic growth of young midbrain neurons, and we have searched for the role of interactive proteins and proteoglycans in this model. Neurite production in co-cultures reveals that, irrespective of the previous location of neurons in the midbrain, medial astrocytes exert an inhibitory or nonpermissive effect on neuritic growth that is correlated to a higher content of both heparan and chondroitin sulfates... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Astrocytes; Axonal growth; Chondroitin sulfate; Heparan sulfate; Midline; Midbrain. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800005 |
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